Biological Waste Guidance

Biological waste includes materials contaminated with potentially infectious biological agents generated from research, teaching, clinical, or veterinary activities. Waste must be segregated into solid, liquid, animal, recombinant, and contaminated glass streams and managed in accordance with approved decontamination and disposal procedures. Refer to the table below for specifics. Note: If Disposal pathway refers to EHS pickup a work order must be submitted through the Physical Plant stating as such.

Biological Waste Classification Table

Waste CategoryExamplesRequired Handling/TreatmentDisposal Pathway
Solid Biohazardous WastePetri dishes, culture plates, contaminated gloves, gowns, bench paper, pipette tips, wipes, culture tubes, absorbent pads, contaminated PPEMust be collected in labeled biohazard bags (red or approved autoclave bags). Treat via autoclave or approved decontamination prior to disposal.landfill disposal (after treatment) OR EHS pickup (as applicable)
Liquid Biohazardous WasteBlood, body fluids, cell culture media, microbial broth cultures, aspirated cell culture waste, wash solutions from biological workMust be chemically disinfected (e.g., 10% bleach or approved disinfectant) OR autoclaved prior to drain disposalSanitary sewer (after treatment) OR EHS pickup (as applicable)
Animal Biological WasteAnimal carcasses, tissues, organs, anatomical remains, infectious animal materials, teaching specimensMust be double-bagged, sealed, and stored frozen if required. Must follow IACUC and biosafety proceduresContact IACUC for coordination of disposal
Human Anatomical/Clinical WasteHuman cadaver tissue, organs, dissection specimens, preserved anatomical material, blood and body fluids from anatomy teaching labs, surgical teaching materialsMust be treated as high-risk pathological waste. Requires containment in leak-proof, labeled biohazard containers or anatomical waste containers. Must be stored under controlled conditions (often refrigeration or chemical preservation depending on program). Must never be disposed of via regular biohazard bags or drain disposal.Must be collected by approved EHS Contractor.
Recombinant/GMO WasteGenetically modified organisms, transgenic plants/animals, recombinant microbial cultures, gene-editing experiment materialsMust follow approved biosafety protocols corresponding to the assigned biosafety level (BSL) and IBC-approved procedures.Autoclave disposal stream or EHS pickup (as applicable)
Contaminated Biological GlasswareBroken culture tubes, Pasteur pipettes contaminated with biological material, non-sharp contaminated glass itemsMust be placed in rigid, labeled biohazard glass containers. May require autoclave prior to disposalBroken glass box after decontamination or EHS pickup (as applicable)
Mixed Biological WasteWaste contaminated with multiple biological agents or requiring enhanced containment proceduresMust follow highest applicable Biosafety Level requirements. May require sequential decontamination stepscase-by-case contact IBC and/or EHS for guidance

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